@article {53, title = {Polygenic adaptation on height is overestimated due to uncorrected stratification in genome-wide association studies.}, journal = {Elife}, volume = {8}, year = {2019}, month = {2019 Mar 21}, abstract = {

Genetic predictions of height differ among human populations and these differences have been interpreted as evidence of polygenic adaptation. These differences were first detected using SNPs genome-wide significantly associated with height, and shown to grow stronger when large numbers of sub-significant SNPs were included, leading to excitement about the prospect of analyzing large fractions of the genome to detect polygenic adaptation for multiple traits. Previous studies of height have been based on SNP effect size measurements in the GIANT Consortium meta-analysis. Here we repeat the analyses in the UK Biobank, a much more homogeneously designed study. We show that polygenic adaptation signals based on large numbers of SNPs below genome-wide significance are extremely sensitive to biases due to uncorrected population stratification. More generally, our results imply that typical constructions of polygenic scores are sensitive to population stratification and that population-level differences should be interpreted with caution.

Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor{\textquoteright}s assessment is that all the issues have been addressed (see decision letter).

}, issn = {2050-084X}, doi = {10.7554/eLife.39702}, author = {Sohail, Mashaal and Maier, Robert M and Ganna, Andrea and Bloemendal, Alex and Martin, Alicia R and Turchin, Michael C and Chiang, Charleston Wk and Hirschhorn, Joel and Daly, Mark J and Patterson, Nick and Neale, Benjamin and Mathieson, Iain and Reich, David and Sunyaev, Shamil R} } @article {40, title = {Functional architecture of low-frequency variants highlights strength of negative selection across coding and non-coding annotations.}, journal = {Nat Genet}, volume = {50}, year = {2018}, month = {2018 11}, pages = {1600-1607}, abstract = {

Common variant heritability has been widely reported to be concentrated in variants within cell-type-specific non-coding functional annotations, but little is known about low-frequency variant functional architectures. We partitioned the heritability of both low-frequency (0.5\%<= minor allele frequency <5\%) and common (minor allele frequency >=5\%) variants in 40 UK Biobank traits across a broad set of functional annotations. We determined that non-synonymous coding variants explain 17 {\textpm} 1\% of low-frequency variant heritability ([Formula: see text]) versus 2.1 {\textpm} 0.2\% of common variant heritability ([Formula: see text]). Cell-type-specific non-coding annotations that were significantly enriched for [Formula: see text] of corresponding traits were similarly enriched for [Formula: see text] for most traits, but more enriched for brain-related annotations and traits. For example, H3K4me3 marks in brain dorsolateral prefrontal cortex explain 57 {\textpm} 12\% of [Formula: see text] versus 12 {\textpm} 2\% of [Formula: see text] for neuroticism. Forward simulations confirmed that low-frequency variant enrichment depends on the mean selection coefficient of causal variants in the annotation, and can be used to predict effect size variance of causal rare variants (minor allele frequency <0.5\%).

}, keywords = {Alleles, Biological Specimen Banks, European Continental Ancestry Group, Gene Frequency, Genetic Variation, Genetics, Population, Genome-Wide Association Study, Humans, Linkage Disequilibrium, Molecular Sequence Annotation, Open Reading Frames, Polymorphism, Single Nucleotide, Selection, Genetic, United Kingdom}, issn = {1546-1718}, doi = {10.1038/s41588-018-0231-8}, author = {Gazal, Steven and Loh, Po-Ru and Finucane, Hilary K and Ganna, Andrea and Schoech, Armin and Sunyaev, Shamil and Price, Alkes L} } @article {30, title = {The impact of rare variation on gene expression across tissues.}, journal = {Nature}, volume = {550}, year = {2017}, month = {2017 10 11}, pages = {239-243}, abstract = {

Rare genetic variants are abundant in humans and are expected to contribute to individual disease risk. While genetic association studies have successfully identified common genetic variants associated with susceptibility, these studies are not practical for identifying rare variants. Efforts to distinguish pathogenic variants from benign rare variants have leveraged the genetic code to identify deleterious protein-coding alleles, but no analogous code exists for non-coding variants. Therefore, ascertaining which rare variants have phenotypic effects remains a major challenge. Rare non-coding variants have been associated with extreme gene expression in studies using single tissues, but their effects across tissues are unknown. Here we identify gene expression outliers, or individuals showing extreme expression levels for a particular gene, across 44 human tissues by using combined analyses of whole genomes and multi-tissue RNA-sequencing data from the Genotype-Tissue Expression (GTEx) project v6p release. We find that 58\% of underexpression and 28\% of overexpression outliers have nearby conserved rare variants compared to 8\% of non-outliers. Additionally, we developed RIVER (RNA-informed variant effect on regulation), a Bayesian statistical model that incorporates expression data to predict a regulatory effect for rare variants with higher accuracy than models using genomic annotations alone. Overall, we demonstrate that rare variants contribute to large gene expression changes across tissues and provide an integrative method for interpretation of rare variants in individual genomes.

}, keywords = {Bayes Theorem, Female, Gene Expression Profiling, Genetic Variation, Genome, Human, Genomics, Genotype, Humans, Male, Models, Genetic, Organ Specificity, Sequence Analysis, RNA}, issn = {1476-4687}, doi = {10.1038/nature24267}, author = {Li, Xin and Kim, Yungil and Tsang, Emily K and Davis, Joe R and Damani, Farhan N and Chiang, Colby and Hess, Gaelen T and Zappala, Zachary and Strober, Benjamin J and Scott, Alexandra J and Li, Amy and Ganna, Andrea and Bassik, Michael C and Merker, Jason D and Hall, Ira M and Battle, Alexis and Montgomery, Stephen B} }